Paul L. Dubin
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Purdue School of Science Research Award, 1993.
Research
The interaction of DNA with nucleic acid binding proteins in transcription and storage is one example of in vivo association between proteins and polyelectrolytes. The biological anionic polysaccharides heparin, hyaluronic acid and chondroitin sulfate also bind proteins, but less is known about binding mechanism or energetics. These questions, as well as the use of synthetic polyelectrolytes to fractionate and immobilize proteins, motivates our work in the area of protein-polyelectrolyte complexes. We use light scattering, electrophoresis, fluorescence spectroscopy and capillary electrophoresis to characterize the structure of soluble protein-polyelectrolyte complexes, and we couple these results to computer modeling.
Similar techniques are being applied to other systems in which polyelectrolytes interact with small colloids: surfactant micelles, and dendrimers. The results are relevant to technological problems in the stabilization and flocculation of industrial dispersions and pharmaceutical/cosmetic formulations.
We are examining separations of proteins and smaller molecules
by size-exclusion chromatography, ion-exchange chromatography, and
by capillary electrophoresis. The focus of this work is on the role
of solute geometry and electrostatic interactions in determining
retention.
Publications
"The Roles of Electrostatic Interaction and Polymer Structure in the "Compositional Heterogeneity in Nonionic/Anionic Mixed Micelles Observed by Frontal Analysis Continuous Capillary Electrophoresis" Langmuir18,
4605-4609 (2002) (with B. Zhang and G.F. Kirton)."Identification by Integrated Computer Modeling and Light Scattering Studies of an Electrostatic Serum Albumin-Hyaluronic Acid Binding Site", Biomacromolecules 2 422-429 (2001). (with K. Grymonpré, B. Staggemeir, and
K.W. Mattison)."Adsorptive Partitioning of an Organic Compound onto Polyelectrolyte-Immobilized Micelles on Porous Glass and Sand" Environmental Science and Technology, 35 2608-2611 (2001) (with Y. Wang, J. Banziger, G.Filipelli and N. Nuraje),
"Binding of Bovine Serum Albumin to Heparin Determined by Turbidimetric Titration and Frontal Analysis Continuous Capillary Electrophoresis", Analytical Biochemistry, 295, 158-167 (2001) (with T. Hattori, K. Kimura and
E. Seyrek)."Interaction of DNA with Cationic Micelles: Effects of Micelle Surface Charge Density, Micelle Shape and Ionic Strength on Complexation and DNA Collapse", Langmuir 17 1670-1673 (2001) with Y. Wang and H. Zhang).
"Counterion Binding on Charged Spheres: Effect of pH and Ionic Strength on the Mobility of Carboxyl-terminated Dendrimers, J. Phys. Chem B 104, 898-904 (2000) (with Q.R. Huang, C.N. Moorefield and G.R. Newkome).
Binding of b-Lactoglobulin to Anionic Polyelectrolytes: Measurement of Binding Constants by FACCE", Langmuir16 9738-9743 (2000) (with T. Hattori and R. Hallberg).
"Small Angle Neutron Scattering of Charged Carboxyl-terminated Dendrimers in Solution", submitted, J. Phys. Chem. (with W. R. Huang, J. Lal, C.N. Moorefield and G.R. Newkome).
"Coacervation of Bovine Serum Albumin with Cationic Polyelectrolytes", submitted, BioMacromolecules, (with K. Kaibara, T. Okazaki, and H.P. Bohidar).
“Determination of the Compositional Distribution of Copolymers by Frontal Analysis Continuous Capillary Electrophoresis”, Analytical Chemistry, 72(1), 255-258 (2000) (with B. Staggemeier, Q. R. Huang, Y. Morishima and T. Sato).
“Counterion Binding on Charged Spheres: Effect of pH and Ionic Strength on the Mobility of Carboxyl-Terminated Dendrimers”, The Journal of Physical Chemistry B, 104(5), 898-904 (2000)(with Q. R. Huang, C. N. Moorefield and G. R. Newkome).
"Light scattering, circular dichroism and Ligand Binding Studies of Hemoglobin-Polyelectrolyte Complexes", Biopolymers, 50, 153 (1999) (with J. Xia, B. Muhoberac*, E. Kokufuta, and H. Havel).
"Capillary Modification by Noncovalent Polycation Adsorption: Effects of Polymer Molecular Weight and Adsorption Ionic Strength" Anal. Chem., 71, 3463-8 (1999) (with Y. Wang)
"Effects of Salt on Polyelectrolyte-Micelle Coacervation", Macromolecules; 32, 7128-7134 (1999) (with Y. Wang, K. Kimura, Q.R. Huang and W. Jaeger)
“Formation of an intrapolymer complex from human serum albumin and poly(ethyleneglycol)”, Langmuir, 15 (4), 940-947 (1999) (with S. Azegami, a. Tsuboi, T. Izumi, M. Hirata, B. Wang and E. Kokufuta).
"Complex Formation by Electrostatic Interaction between Carboxyl-terminated Dendrimer and Oppositely Charged Polyelectrolyte", submitted, Langmuir (N. Miura, P. L. Dubin, C.M. Moorefield and G. Newkome).
"Binding of Proteins to Copolymers of Varying Hydrophobicity",Biopolymers, 49, 185-193, (J. Gao and P. L. Dubin).
"Interaction of a Polycation with Small Oppositely Charged Dendrimers", J. Phys. Chem., J. Phys. Chem., 103, 2347-2354 (1999) (H. Zhang, J. Ray, P. L. Dubin, G.S. Manning, G.R. Newkome and C.S. Moorefield).
"Effect of pH on the Binding of B-lactoglobulin to Sodium Polystyrene Sulfonate", J. Phys. Chem.,
102 8629-8633 (1998) (with R. Hallberg and P. L. Dubin)."Protein Binding on Polyelectrolyte-treated Glass: Effect of Microscopic Structure of Adsorbed Polyelectrolyte", J. Chromatogr., 808 61-70 (1998) (Y. Wang and P. L. Dubin).
"Binding of Polycarboxylic Acids to Cationic Mixed Micelles: Effects of Polymer Counterion Binding and Polyion Charge Distribution", J. Colloid Interface Sci., 205, 257-264 (1998) (K. Yoshida, S. Sokhakian and P. L. Dubin).
"Permeation of Small Molecules in Aqueous Size-Exclusion Chromatography vis-a-vis Models for Separation", Analytical Chemistry 70, 1576-1580 (1998) (H. Dai P. L. Dubin and T. Andersson).
"Fluorescence Quenching Studies of the Interaction of a Pyrene-labeled Polyelectrolyte with Quencher-Carrying Oppositely Charged Micelles", Langmuir, 13, 6941-6 (1997) (with M. Mizusaki, Y. Morishima, P. L. Dubin and K. Yoshida).
"Dissociation of Carboxyl-terminated Cascade Polymers: Comparison with Theory", J. Phys. Chem., 101, 3494-3497 (1997), ( H. Zhang, P. L. Dubin, J.I. Kaplan, C.N. Moorefield and G.R. Newkome).
"Measurement of the Binding of Proteins to Polyelectrolytes by Frontal Analysis Continuous Capillary Electrophoresis, Anal. Chem. 69, 2945-2951 (1997)(Y. Gao, P. L. Dubin, B. B. Muhobenac).
