Nano Imaging Center

Instrumentation

Confocal Fluorescence Correlation Spectroscopy System (Confocor 2, Zeiss):

The Confocor 2 is equipped with an Argon laser (458, 488, 514nm excitation) and two Helium-Neon laser (543 and 633nm excitation). Two independent detector channels with fiber-coupled Avalanche Photodiodes (APD) allow the simultaneous measurements of two autocorrelation curves and one cross correlation curve. The system involves a Zeiss inverted microscope Axiovert 200M, which can be operated in epifluorescence, bright-field, phase-contrast, and DIC modes. Computer-controlled image acquisition is feasible using a CoolSNAPfx CCD camera.

Carl Zeiss, Inc.

Wide-field Single Molecule Fluorescence Microscopy:

The main components of this custom-built imaging system are a Crystalaser 200mW frequency doubled Nd:YAG laser (532nm excitation), an inverted microscope (Zeiss, Axiovert100 TV) and an extremely sensitive intensified CCD camera (iPentamex 512EFT). The imaging system is capable of tracking individual fluorophores with millisecond resolution using an automated particle tracking software (ISee).

Near-field Scanning Optical Microscopy (NSOM):

The near-field imaging system is based on a Helium-Neon laser, a laser-to-fiber coupler, a single mode optical fiber linked to an NSOM probe, a piezo stage, and a sensitive photon detector. The small diameter of the aperture of the NSOM probe facilitates imaging below the diffraction limit of conventional wide-field optical microscopy.

Combined Atomic Force and Fluorescence Microscopy (BioScope, Veeco):

The BioScope represents a full atomic force microscope (AFM) on a scientific inverted optical microscope (Axiovert 135, Zeiss). This combination of imaging equipment allows for the nondestructive scanning with familiar sample preparation, mounting, and optical capabilities. The AFM provides resolution on the nanometer (lateral) and angstrom (vertical) scales, which is comparable to electron microscopy (EM). The AFM can be operated in several imaging modes, including contact mode, tapping mode, lateral force microscopy, phase imaging, and magnetic force microscopy. The inverted optical microscope allows for parallel standard epifluroscence imaging.

Other Recent Equipment Additions:

Perkin Elmer Spectrum-One FTIR Scanning Microscope
Craig Technologies QDI 2000 Microscope
SLM/Aminco 8000C Spectrofluorometer with ISS upgrade


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IUPUI Main IUPUI Chemistry IUPUI School of Science IU-Bloomington Purdue University	Instrumentation Confocal Fluorescence Correlation Spectroscopy System (Confocor 2, Zeiss): The Confocor 2 is equipped with an Argon laser (458, 488, 514nm excitation) and two Helium-Neon laser (543 and 633nm excitation). Two independent detector channels with fiber-coupled Avalanche Photodiodes (APD) allow the simultaneous measurements of two autocorrelation curves and one cross correlation curve. The system involves a Zeiss inverted microscope Axiovert 200M, which can be operated in epifluorescence, bright-field, phase-contrast, and DIC modes. Computer-controlled image acquisition is feasible using a CoolSNAPfx CCD camera. Carl Zeiss, Inc. Wide-field Single Molecule Fluorescence Microscopy: The main components of this custom-built imaging system are a Crystalaser 200mW frequency doubled Nd:YAG laser (532nm excitation), an inverted microscope (Zeiss, Axiovert100 TV) and an extremely sensitive intensified CCD camera (iPentamex 512EFT). The imaging system is capable of tracking individual fluorophores with millisecond resolution using an automated particle tracking software (ISee). Near-field Scanning Optical Microscopy (NSOM): The near-field imaging system is based on a Helium-Neon laser, a laser-to-fiber coupler, a single mode optical fiber linked to an NSOM probe, a piezo stage, and a sensitive photon detector. The small diameter of the aperture of the NSOM probe facilitates imaging below the diffraction limit of conventional wide-field optical microscopy. Combined Atomic Force and Fluorescence Microscopy (BioScope, Veeco): The BioScope represents a full atomic force microscope (AFM) on a scientific inverted optical microscope (Axiovert 135, Zeiss). This combination of imaging equipment allows for the nondestructive scanning with familiar sample preparation, mounting, and optical capabilities. The AFM provides resolution on the nanometer (lateral) and angstrom (vertical) scales, which is comparable to electron microscopy (EM). The AFM can be operated in several imaging modes, including contact mode, tapping mode, lateral force microscopy, phase imaging, and magnetic force microscopy. The inverted optical microscope allows for parallel standard epifluroscence imaging. Other Recent Equipment Additions: Perkin Elmer Spectrum-One FTIR Scanning Microscope Craig Technologies QDI 2000 Microscope SLM/Aminco 8000C Spectrofluorometer with ISS upgrade
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